4d-nucleofector x unit aaf-1003x (Lonza)
Lonza is a verified supplier 
Lonza manufactures this product 
90
Structured Review
Lonza
4d-nucleofector x unit aaf-1003x
4d Nucleofector X Unit Aaf 1003x, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4d-nucleofector+x+unit/4d+nucleofector/pmc12272864-50-6-4
Average 90 stars, based on 1 article reviews
4d Nucleofector X Unit Aaf 1003x, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/4d-nucleofector+x+unit/4d+nucleofector/pmc12272864-50-6-4
Average 90 stars, based on 1 article reviews
4d-nucleofector x unit aaf-1003x - by Bioz Stars,
2026-09
90/100 stars
Images
Related Articles
Microscopy:Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells Article Snippet: The reactions were performed using the “CB-150” program on the Article Title: Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling. Article Snippet: Article Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling Highlights • IL-15R expression regulates cytotoxic immune cell function • CRISPR screens reveal IL-15R signaling inhibition by CRL5dependent degradation • UBE2F deletion inhibits CRL5 activity, increasing NK cell responsiveness to IL-15 • UBE2F loss enhances CAR NK cells and endogenous solid tumor immunity in mice Authors Iva Nikolic, Joseph Cursons, Benjamin Shields, ..., Richard Berry, Jai Rautela, Nicholas D. Huntington Correspondence jai@onko-innate.com (J.R.), nicholas.huntington@monash.edu (N.D.H.). In brief Tumor microenvironment IL-15 can regulate anti-tumor immunity.. In this study, Nikolic et al. perform genome-wide CRISPR screening in natural killer cells and discover that UBE2F-dependent activation of the Cullin-5 RING E3 ligase (CRL5) complex results in IL-15R degradation and signaling inhibition. Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors were mixed at a mass ratio of 4:1 and transfected into hiPSCs using Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC. Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors weremixed at amass ratio of 4:1 and transfected into hiPSCs using Article Title: Genetic variation reveals a homeotic long noncoding RNA that modulates human hematopoietic stem cells Article Snippet: Cells (5×10 5 ) were electroporated using the Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization Article Snippet: The mixture was introduced into the cells using the Article Title: A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells. Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER UCSC Genome Browser on Human (GRCh38/hg38) Perez et al. 7 genome.ucsc.edu Other 4D-Nucleofector X unit Lonza AAF-1003X Stereo microscope Nikon MBW10020 Biosafety cabinet Baker Sterilgard WOLF G2 benchtop microfluidic cell sorter NanoCellect N/A Thermocycler Applied Biosystems VeritiPro Benchtop centrifuge for the PCR tubes ISC BioExpress C1301 0.2 μL PCR tubes Applied Biosystems N8010535 Cryovials (1 mL) Greiner 122279 37 ◦ C - 5% CO 2 tissue culture incubator Thermo Fisher Scientific HERAcell Vios 160i 6-well plates Nunc 140675 10 cm plates Nunc 140464 18 G hypodermic needles Fisher Scientific 14-826-5D CloneR medium Reagent Final concentration Amount mTeSR Plus Medium 1X 45 mL CloneR 1X 5 mL Total N/A 50 mL Store at 4 ◦ C for up to 7 days. Hood:Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells Article Snippet: The reactions were performed using the “CB-150” program on the Article Title: Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling. Article Snippet: Article Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling Highlights • IL-15R expression regulates cytotoxic immune cell function • CRISPR screens reveal IL-15R signaling inhibition by CRL5dependent degradation • UBE2F deletion inhibits CRL5 activity, increasing NK cell responsiveness to IL-15 • UBE2F loss enhances CAR NK cells and endogenous solid tumor immunity in mice Authors Iva Nikolic, Joseph Cursons, Benjamin Shields, ..., Richard Berry, Jai Rautela, Nicholas D. Huntington Correspondence jai@onko-innate.com (J.R.), nicholas.huntington@monash.edu (N.D.H.). In brief Tumor microenvironment IL-15 can regulate anti-tumor immunity.. In this study, Nikolic et al. perform genome-wide CRISPR screening in natural killer cells and discover that UBE2F-dependent activation of the Cullin-5 RING E3 ligase (CRL5) complex results in IL-15R degradation and signaling inhibition. Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors were mixed at a mass ratio of 4:1 and transfected into hiPSCs using Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC. Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors weremixed at amass ratio of 4:1 and transfected into hiPSCs using Article Title: Genetic variation reveals a homeotic long noncoding RNA that modulates human hematopoietic stem cells Article Snippet: Cells (5×10 5 ) were electroporated using the Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization Article Snippet: The mixture was introduced into the cells using the Article Title: A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells. Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER UCSC Genome Browser on Human (GRCh38/hg38) Perez et al. 7 genome.ucsc.edu Other 4D-Nucleofector X unit Lonza AAF-1003X Stereo microscope Nikon MBW10020 Biosafety cabinet Baker Sterilgard WOLF G2 benchtop microfluidic cell sorter NanoCellect N/A Thermocycler Applied Biosystems VeritiPro Benchtop centrifuge for the PCR tubes ISC BioExpress C1301 0.2 μL PCR tubes Applied Biosystems N8010535 Cryovials (1 mL) Greiner 122279 37 ◦ C - 5% CO 2 tissue culture incubator Thermo Fisher Scientific HERAcell Vios 160i 6-well plates Nunc 140675 10 cm plates Nunc 140464 18 G hypodermic needles Fisher Scientific 14-826-5D CloneR medium Reagent Final concentration Amount mTeSR Plus Medium 1X 45 mL CloneR 1X 5 mL Total N/A 50 mL Store at 4 ◦ C for up to 7 days. Polymerase Chain Reaction:Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells Article Snippet: The reactions were performed using the “CB-150” program on the Article Title: Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling. Article Snippet: Article Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling Highlights • IL-15R expression regulates cytotoxic immune cell function • CRISPR screens reveal IL-15R signaling inhibition by CRL5dependent degradation • UBE2F deletion inhibits CRL5 activity, increasing NK cell responsiveness to IL-15 • UBE2F loss enhances CAR NK cells and endogenous solid tumor immunity in mice Authors Iva Nikolic, Joseph Cursons, Benjamin Shields, ..., Richard Berry, Jai Rautela, Nicholas D. Huntington Correspondence jai@onko-innate.com (J.R.), nicholas.huntington@monash.edu (N.D.H.). In brief Tumor microenvironment IL-15 can regulate anti-tumor immunity.. In this study, Nikolic et al. perform genome-wide CRISPR screening in natural killer cells and discover that UBE2F-dependent activation of the Cullin-5 RING E3 ligase (CRL5) complex results in IL-15R degradation and signaling inhibition. Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors were mixed at a mass ratio of 4:1 and transfected into hiPSCs using Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC. Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors weremixed at amass ratio of 4:1 and transfected into hiPSCs using Article Title: Genetic variation reveals a homeotic long noncoding RNA that modulates human hematopoietic stem cells Article Snippet: Cells (5×10 5 ) were electroporated using the Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization Article Snippet: The mixture was introduced into the cells using the Article Title: A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells. Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER UCSC Genome Browser on Human (GRCh38/hg38) Perez et al. 7 genome.ucsc.edu Other 4D-Nucleofector X unit Lonza AAF-1003X Stereo microscope Nikon MBW10020 Biosafety cabinet Baker Sterilgard WOLF G2 benchtop microfluidic cell sorter NanoCellect N/A Thermocycler Applied Biosystems VeritiPro Benchtop centrifuge for the PCR tubes ISC BioExpress C1301 0.2 μL PCR tubes Applied Biosystems N8010535 Cryovials (1 mL) Greiner 122279 37 ◦ C - 5% CO 2 tissue culture incubator Thermo Fisher Scientific HERAcell Vios 160i 6-well plates Nunc 140675 10 cm plates Nunc 140464 18 G hypodermic needles Fisher Scientific 14-826-5D CloneR medium Reagent Final concentration Amount mTeSR Plus Medium 1X 45 mL CloneR 1X 5 mL Total N/A 50 mL Store at 4 ◦ C for up to 7 days. Concentration Assay:Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells Article Snippet: The reactions were performed using the “CB-150” program on the Article Title: Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling. Article Snippet: Article Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling Highlights • IL-15R expression regulates cytotoxic immune cell function • CRISPR screens reveal IL-15R signaling inhibition by CRL5dependent degradation • UBE2F deletion inhibits CRL5 activity, increasing NK cell responsiveness to IL-15 • UBE2F loss enhances CAR NK cells and endogenous solid tumor immunity in mice Authors Iva Nikolic, Joseph Cursons, Benjamin Shields, ..., Richard Berry, Jai Rautela, Nicholas D. Huntington Correspondence jai@onko-innate.com (J.R.), nicholas.huntington@monash.edu (N.D.H.). In brief Tumor microenvironment IL-15 can regulate anti-tumor immunity.. In this study, Nikolic et al. perform genome-wide CRISPR screening in natural killer cells and discover that UBE2F-dependent activation of the Cullin-5 RING E3 ligase (CRL5) complex results in IL-15R degradation and signaling inhibition. Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors were mixed at a mass ratio of 4:1 and transfected into hiPSCs using Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC. Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors weremixed at amass ratio of 4:1 and transfected into hiPSCs using Article Title: Genetic variation reveals a homeotic long noncoding RNA that modulates human hematopoietic stem cells Article Snippet: Cells (5×10 5 ) were electroporated using the Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization Article Snippet: The mixture was introduced into the cells using the Article Title: A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells. Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER UCSC Genome Browser on Human (GRCh38/hg38) Perez et al. 7 genome.ucsc.edu Other 4D-Nucleofector X unit Lonza AAF-1003X Stereo microscope Nikon MBW10020 Biosafety cabinet Baker Sterilgard WOLF G2 benchtop microfluidic cell sorter NanoCellect N/A Thermocycler Applied Biosystems VeritiPro Benchtop centrifuge for the PCR tubes ISC BioExpress C1301 0.2 μL PCR tubes Applied Biosystems N8010535 Cryovials (1 mL) Greiner 122279 37 ◦ C - 5% CO 2 tissue culture incubator Thermo Fisher Scientific HERAcell Vios 160i 6-well plates Nunc 140675 10 cm plates Nunc 140464 18 G hypodermic needles Fisher Scientific 14-826-5D CloneR medium Reagent Final concentration Amount mTeSR Plus Medium 1X 45 mL CloneR 1X 5 mL Total N/A 50 mL Store at 4 ◦ C for up to 7 days. Flow Cytometry:Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells Article Snippet: The reactions were performed using the “CB-150” program on the Article Title: Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling. Article Snippet: Article Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling Highlights • IL-15R expression regulates cytotoxic immune cell function • CRISPR screens reveal IL-15R signaling inhibition by CRL5dependent degradation • UBE2F deletion inhibits CRL5 activity, increasing NK cell responsiveness to IL-15 • UBE2F loss enhances CAR NK cells and endogenous solid tumor immunity in mice Authors Iva Nikolic, Joseph Cursons, Benjamin Shields, ..., Richard Berry, Jai Rautela, Nicholas D. Huntington Correspondence jai@onko-innate.com (J.R.), nicholas.huntington@monash.edu (N.D.H.). In brief Tumor microenvironment IL-15 can regulate anti-tumor immunity.. In this study, Nikolic et al. perform genome-wide CRISPR screening in natural killer cells and discover that UBE2F-dependent activation of the Cullin-5 RING E3 ligase (CRL5) complex results in IL-15R degradation and signaling inhibition. Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors were mixed at a mass ratio of 4:1 and transfected into hiPSCs using Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC. Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors weremixed at amass ratio of 4:1 and transfected into hiPSCs using Article Title: Genetic variation reveals a homeotic long noncoding RNA that modulates human hematopoietic stem cells Article Snippet: Cells (5×10 5 ) were electroporated using the Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization Article Snippet: The mixture was introduced into the cells using the Article Title: A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells. Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER UCSC Genome Browser on Human (GRCh38/hg38) Perez et al. 7 genome.ucsc.edu Other 4D-Nucleofector X unit Lonza AAF-1003X Stereo microscope Nikon MBW10020 Biosafety cabinet Baker Sterilgard WOLF G2 benchtop microfluidic cell sorter NanoCellect N/A Thermocycler Applied Biosystems VeritiPro Benchtop centrifuge for the PCR tubes ISC BioExpress C1301 0.2 μL PCR tubes Applied Biosystems N8010535 Cryovials (1 mL) Greiner 122279 37 ◦ C - 5% CO 2 tissue culture incubator Thermo Fisher Scientific HERAcell Vios 160i 6-well plates Nunc 140675 10 cm plates Nunc 140464 18 G hypodermic needles Fisher Scientific 14-826-5D CloneR medium Reagent Final concentration Amount mTeSR Plus Medium 1X 45 mL CloneR 1X 5 mL Total N/A 50 mL Store at 4 ◦ C for up to 7 days. Chromogenic In Situ Hybridization:Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells Article Snippet: The reactions were performed using the “CB-150” program on the Article Title: Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling. Article Snippet: Article Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling Highlights • IL-15R expression regulates cytotoxic immune cell function • CRISPR screens reveal IL-15R signaling inhibition by CRL5dependent degradation • UBE2F deletion inhibits CRL5 activity, increasing NK cell responsiveness to IL-15 • UBE2F loss enhances CAR NK cells and endogenous solid tumor immunity in mice Authors Iva Nikolic, Joseph Cursons, Benjamin Shields, ..., Richard Berry, Jai Rautela, Nicholas D. Huntington Correspondence jai@onko-innate.com (J.R.), nicholas.huntington@monash.edu (N.D.H.). In brief Tumor microenvironment IL-15 can regulate anti-tumor immunity.. In this study, Nikolic et al. perform genome-wide CRISPR screening in natural killer cells and discover that UBE2F-dependent activation of the Cullin-5 RING E3 ligase (CRL5) complex results in IL-15R degradation and signaling inhibition. Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors were mixed at a mass ratio of 4:1 and transfected into hiPSCs using Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC. Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors weremixed at amass ratio of 4:1 and transfected into hiPSCs using Article Title: Genetic variation reveals a homeotic long noncoding RNA that modulates human hematopoietic stem cells Article Snippet: Cells (5×10 5 ) were electroporated using the Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization Article Snippet: The mixture was introduced into the cells using the Article Title: A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells. Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER UCSC Genome Browser on Human (GRCh38/hg38) Perez et al. 7 genome.ucsc.edu Other 4D-Nucleofector X unit Lonza AAF-1003X Stereo microscope Nikon MBW10020 Biosafety cabinet Baker Sterilgard WOLF G2 benchtop microfluidic cell sorter NanoCellect N/A Thermocycler Applied Biosystems VeritiPro Benchtop centrifuge for the PCR tubes ISC BioExpress C1301 0.2 μL PCR tubes Applied Biosystems N8010535 Cryovials (1 mL) Greiner 122279 37 ◦ C - 5% CO 2 tissue culture incubator Thermo Fisher Scientific HERAcell Vios 160i 6-well plates Nunc 140675 10 cm plates Nunc 140464 18 G hypodermic needles Fisher Scientific 14-826-5D CloneR medium Reagent Final concentration Amount mTeSR Plus Medium 1X 45 mL CloneR 1X 5 mL Total N/A 50 mL Store at 4 ◦ C for up to 7 days. Software:Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells Article Snippet: The reactions were performed using the “CB-150” program on the Article Title: Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling. Article Snippet: Article Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling Highlights • IL-15R expression regulates cytotoxic immune cell function • CRISPR screens reveal IL-15R signaling inhibition by CRL5dependent degradation • UBE2F deletion inhibits CRL5 activity, increasing NK cell responsiveness to IL-15 • UBE2F loss enhances CAR NK cells and endogenous solid tumor immunity in mice Authors Iva Nikolic, Joseph Cursons, Benjamin Shields, ..., Richard Berry, Jai Rautela, Nicholas D. Huntington Correspondence jai@onko-innate.com (J.R.), nicholas.huntington@monash.edu (N.D.H.). In brief Tumor microenvironment IL-15 can regulate anti-tumor immunity.. In this study, Nikolic et al. perform genome-wide CRISPR screening in natural killer cells and discover that UBE2F-dependent activation of the Cullin-5 RING E3 ligase (CRL5) complex results in IL-15R degradation and signaling inhibition. Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors were mixed at a mass ratio of 4:1 and transfected into hiPSCs using Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC. Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors weremixed at amass ratio of 4:1 and transfected into hiPSCs using Article Title: Genetic variation reveals a homeotic long noncoding RNA that modulates human hematopoietic stem cells Article Snippet: Cells (5×10 5 ) were electroporated using the Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization Article Snippet: The mixture was introduced into the cells using the Article Title: A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells. Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER UCSC Genome Browser on Human (GRCh38/hg38) Perez et al. 7 genome.ucsc.edu Other 4D-Nucleofector X unit Lonza AAF-1003X Stereo microscope Nikon MBW10020 Biosafety cabinet Baker Sterilgard WOLF G2 benchtop microfluidic cell sorter NanoCellect N/A Thermocycler Applied Biosystems VeritiPro Benchtop centrifuge for the PCR tubes ISC BioExpress C1301 0.2 μL PCR tubes Applied Biosystems N8010535 Cryovials (1 mL) Greiner 122279 37 ◦ C - 5% CO 2 tissue culture incubator Thermo Fisher Scientific HERAcell Vios 160i 6-well plates Nunc 140675 10 cm plates Nunc 140464 18 G hypodermic needles Fisher Scientific 14-826-5D CloneR medium Reagent Final concentration Amount mTeSR Plus Medium 1X 45 mL CloneR 1X 5 mL Total N/A 50 mL Store at 4 ◦ C for up to 7 days. Suspension:Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells Article Snippet: The reactions were performed using the “CB-150” program on the Article Title: Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling. Article Snippet: Article Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling Highlights • IL-15R expression regulates cytotoxic immune cell function • CRISPR screens reveal IL-15R signaling inhibition by CRL5dependent degradation • UBE2F deletion inhibits CRL5 activity, increasing NK cell responsiveness to IL-15 • UBE2F loss enhances CAR NK cells and endogenous solid tumor immunity in mice Authors Iva Nikolic, Joseph Cursons, Benjamin Shields, ..., Richard Berry, Jai Rautela, Nicholas D. Huntington Correspondence jai@onko-innate.com (J.R.), nicholas.huntington@monash.edu (N.D.H.). In brief Tumor microenvironment IL-15 can regulate anti-tumor immunity.. In this study, Nikolic et al. perform genome-wide CRISPR screening in natural killer cells and discover that UBE2F-dependent activation of the Cullin-5 RING E3 ligase (CRL5) complex results in IL-15R degradation and signaling inhibition. Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors were mixed at a mass ratio of 4:1 and transfected into hiPSCs using Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC. Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors weremixed at amass ratio of 4:1 and transfected into hiPSCs using Article Title: Genetic variation reveals a homeotic long noncoding RNA that modulates human hematopoietic stem cells Article Snippet: Cells (5×10 5 ) were electroporated using the Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization Article Snippet: The mixture was introduced into the cells using the Article Title: A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells. Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER UCSC Genome Browser on Human (GRCh38/hg38) Perez et al. 7 genome.ucsc.edu Other 4D-Nucleofector X unit Lonza AAF-1003X Stereo microscope Nikon MBW10020 Biosafety cabinet Baker Sterilgard WOLF G2 benchtop microfluidic cell sorter NanoCellect N/A Thermocycler Applied Biosystems VeritiPro Benchtop centrifuge for the PCR tubes ISC BioExpress C1301 0.2 μL PCR tubes Applied Biosystems N8010535 Cryovials (1 mL) Greiner 122279 37 ◦ C - 5% CO 2 tissue culture incubator Thermo Fisher Scientific HERAcell Vios 160i 6-well plates Nunc 140675 10 cm plates Nunc 140464 18 G hypodermic needles Fisher Scientific 14-826-5D CloneR medium Reagent Final concentration Amount mTeSR Plus Medium 1X 45 mL CloneR 1X 5 mL Total N/A 50 mL Store at 4 ◦ C for up to 7 days. Electroporation:Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells Article Snippet: The reactions were performed using the “CB-150” program on the Article Title: Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling. Article Snippet: Article Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling Highlights • IL-15R expression regulates cytotoxic immune cell function • CRISPR screens reveal IL-15R signaling inhibition by CRL5dependent degradation • UBE2F deletion inhibits CRL5 activity, increasing NK cell responsiveness to IL-15 • UBE2F loss enhances CAR NK cells and endogenous solid tumor immunity in mice Authors Iva Nikolic, Joseph Cursons, Benjamin Shields, ..., Richard Berry, Jai Rautela, Nicholas D. Huntington Correspondence jai@onko-innate.com (J.R.), nicholas.huntington@monash.edu (N.D.H.). In brief Tumor microenvironment IL-15 can regulate anti-tumor immunity.. In this study, Nikolic et al. perform genome-wide CRISPR screening in natural killer cells and discover that UBE2F-dependent activation of the Cullin-5 RING E3 ligase (CRL5) complex results in IL-15R degradation and signaling inhibition. Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors were mixed at a mass ratio of 4:1 and transfected into hiPSCs using Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC. Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors weremixed at amass ratio of 4:1 and transfected into hiPSCs using Article Title: Genetic variation reveals a homeotic long noncoding RNA that modulates human hematopoietic stem cells Article Snippet: Cells (5×10 5 ) were electroporated using the Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization Article Snippet: The mixture was introduced into the cells using the Article Title: A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells. Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER UCSC Genome Browser on Human (GRCh38/hg38) Perez et al. 7 genome.ucsc.edu Other 4D-Nucleofector X unit Lonza AAF-1003X Stereo microscope Nikon MBW10020 Biosafety cabinet Baker Sterilgard WOLF G2 benchtop microfluidic cell sorter NanoCellect N/A Thermocycler Applied Biosystems VeritiPro Benchtop centrifuge for the PCR tubes ISC BioExpress C1301 0.2 μL PCR tubes Applied Biosystems N8010535 Cryovials (1 mL) Greiner 122279 37 ◦ C - 5% CO 2 tissue culture incubator Thermo Fisher Scientific HERAcell Vios 160i 6-well plates Nunc 140675 10 cm plates Nunc 140464 18 G hypodermic needles Fisher Scientific 14-826-5D CloneR medium Reagent Final concentration Amount mTeSR Plus Medium 1X 45 mL CloneR 1X 5 mL Total N/A 50 mL Store at 4 ◦ C for up to 7 days. Expressing:Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells Article Snippet: The reactions were performed using the “CB-150” program on the Article Title: Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling. Article Snippet: Article Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling Highlights • IL-15R expression regulates cytotoxic immune cell function • CRISPR screens reveal IL-15R signaling inhibition by CRL5dependent degradation • UBE2F deletion inhibits CRL5 activity, increasing NK cell responsiveness to IL-15 • UBE2F loss enhances CAR NK cells and endogenous solid tumor immunity in mice Authors Iva Nikolic, Joseph Cursons, Benjamin Shields, ..., Richard Berry, Jai Rautela, Nicholas D. Huntington Correspondence jai@onko-innate.com (J.R.), nicholas.huntington@monash.edu (N.D.H.). In brief Tumor microenvironment IL-15 can regulate anti-tumor immunity.. In this study, Nikolic et al. perform genome-wide CRISPR screening in natural killer cells and discover that UBE2F-dependent activation of the Cullin-5 RING E3 ligase (CRL5) complex results in IL-15R degradation and signaling inhibition. Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors were mixed at a mass ratio of 4:1 and transfected into hiPSCs using Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC. Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors weremixed at amass ratio of 4:1 and transfected into hiPSCs using Article Title: Genetic variation reveals a homeotic long noncoding RNA that modulates human hematopoietic stem cells Article Snippet: Cells (5×10 5 ) were electroporated using the Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization Article Snippet: The mixture was introduced into the cells using the Article Title: A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells. Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER UCSC Genome Browser on Human (GRCh38/hg38) Perez et al. 7 genome.ucsc.edu Other 4D-Nucleofector X unit Lonza AAF-1003X Stereo microscope Nikon MBW10020 Biosafety cabinet Baker Sterilgard WOLF G2 benchtop microfluidic cell sorter NanoCellect N/A Thermocycler Applied Biosystems VeritiPro Benchtop centrifuge for the PCR tubes ISC BioExpress C1301 0.2 μL PCR tubes Applied Biosystems N8010535 Cryovials (1 mL) Greiner 122279 37 ◦ C - 5% CO 2 tissue culture incubator Thermo Fisher Scientific HERAcell Vios 160i 6-well plates Nunc 140675 10 cm plates Nunc 140464 18 G hypodermic needles Fisher Scientific 14-826-5D CloneR medium Reagent Final concentration Amount mTeSR Plus Medium 1X 45 mL CloneR 1X 5 mL Total N/A 50 mL Store at 4 ◦ C for up to 7 days. Transfection:Article Title: Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells Article Snippet: The reactions were performed using the “CB-150” program on the Article Title: Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling. Article Snippet: Article Enhancing anti-tumor immunity of natural killer cells through targeting IL-15R signaling Highlights • IL-15R expression regulates cytotoxic immune cell function • CRISPR screens reveal IL-15R signaling inhibition by CRL5dependent degradation • UBE2F deletion inhibits CRL5 activity, increasing NK cell responsiveness to IL-15 • UBE2F loss enhances CAR NK cells and endogenous solid tumor immunity in mice Authors Iva Nikolic, Joseph Cursons, Benjamin Shields, ..., Richard Berry, Jai Rautela, Nicholas D. Huntington Correspondence jai@onko-innate.com (J.R.), nicholas.huntington@monash.edu (N.D.H.). In brief Tumor microenvironment IL-15 can regulate anti-tumor immunity.. In this study, Nikolic et al. perform genome-wide CRISPR screening in natural killer cells and discover that UBE2F-dependent activation of the Cullin-5 RING E3 ligase (CRL5) complex results in IL-15R degradation and signaling inhibition. Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors were mixed at a mass ratio of 4:1 and transfected into hiPSCs using Article Title: Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC. Article Snippet: TF (pBAN-TF-BC) and Super PiggyBac (SPB) Transposase (System Biosciences, PB210PA-1) expression vectors weremixed at amass ratio of 4:1 and transfected into hiPSCs using Article Title: Genetic variation reveals a homeotic long noncoding RNA that modulates human hematopoietic stem cells Article Snippet: Cells (5×10 5 ) were electroporated using the Article Title: Combining light-induced aggregation and biotin proximity labeling implicates endolysosomal proteins in early α-synuclein oligomerization Article Snippet: The mixture was introduced into the cells using the Article Title: A rapid and robust protocol for generating loss-of-function alleles in pluripotent stem cells. Article Snippet: Continued REAGENT or RESOURCE SOURCE IDENTIFIER UCSC Genome Browser on Human (GRCh38/hg38) Perez et al. 7 genome.ucsc.edu Other 4D-Nucleofector X unit Lonza AAF-1003X Stereo microscope Nikon MBW10020 Biosafety cabinet Baker Sterilgard WOLF G2 benchtop microfluidic cell sorter NanoCellect N/A Thermocycler Applied Biosystems VeritiPro Benchtop centrifuge for the PCR tubes ISC BioExpress C1301 0.2 μL PCR tubes Applied Biosystems N8010535 Cryovials (1 mL) Greiner 122279 37 ◦ C - 5% CO 2 tissue culture incubator Thermo Fisher Scientific HERAcell Vios 160i 6-well plates Nunc 140675 10 cm plates Nunc 140464 18 G hypodermic needles Fisher Scientific 14-826-5D CloneR medium Reagent Final concentration Amount mTeSR Plus Medium 1X 45 mL CloneR 1X 5 mL Total N/A 50 mL Store at 4 ◦ C for up to 7 days. |